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| The efficacy of thymosin alpha 1 for severe sepsis |
| Wang Wei，Cen Zhong-ran，Liu Lin-gang，LI Xia-xi，Huang Qiong，Chang Ping |
| Intensive Care Unit, the Fifth Affiliated Hospital of zhengzhou University，Zhengzhou 450052，China |
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Abstract Objective To observe whether thymosin alpha 1（Tα1）improves prognosis and im⁃ mune function of patients with severe sepsis. Methods We retrospectively collected patient information between January 2013 and December 2014 at our department. According to Tα1 treatment，patients with severe sepsis were divided into Tα1 group（n＝127）and non- Tα1 group（n＝117）. Patients in both groups were also stratified according to lymphocyte count on Day 1（the day when patients were diag⁃ nosed with severe sepsis）into the following subgroups: ≥1.00 × 109 lymphocytes/L，（0.50~1.00）×109 lymphocytes/L，and ≤0.50×109 lymphocytes/L. Gender，age，APACHE Ⅱ score，SOFA score，lymphocyte，site of infection causing sepsis and prognosis were retrospectively collected. We performed experi⁃ ments in vitro to observe the effect of Tα1 on immune function in patients with severe sepsis；this was done by evaluating tumor necrosis factor-alpha（TNF-α）levels in peripheral blood mononuclear cells （PBMCs）stimulated by lipopolysaccharide（LPS）. PBMCs from each patient with severe sepsis were di⁃ vided into three groups: the control group，the LPS group，and the LPS+Tα1 group. The concentrations ofTNF-α from each group were tested by ELISA. Results All-cause mortality of patients at 28 days，for both groups，was not significantly different（P＞0.05），and survival time was also similar（P＞0.05）. When patients were stratified by lymphocyte count，all-cause mortality at 28 days for the subgroup with lymphocyte count ≤0.50×109 /L was significantly lower in Tα1 group than non-Tα1 group（P＜0.05）. The survival time of patients in Tα1 group was significantly longer than for those in non-Tα1 group（P＜ 0.05）. In vitro experiments showed markedly higher TNFα levels in the LPS + Tα1 group，compared with the control group and the LPS group（P＜0.05，respectively）. Conclusion The present study indi⁃ cates that Tα1 can significantly improve the prognosis of severe sepsis patients with lymphocyte count ≤ 0.50×109 /L，and improve immune function in patients with severe sepsis.
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Received: 15 March 2016
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Corresponding Authors:
Chang Ping，E-mail: changp9631@163.com
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Address: 1 North 2nd Street,
Zhongguancun, 