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| Protective effect of galangin on the LPS-induced inflammation in THP-1 cells |
| KONG Tian-yu，ZHANG Zhen-hui ，HU-Huan ，GU Hong-jiao ，XIONG Xu-ming. |
| Intensive Care Unit，the Second Affiliated Hospital of Guangzhou Medical University，Guangzhou 510260，China |
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Abstract Objective To observe the protective effect of galangin on the inflammation of human monocytic THP-1 cells induced by lipopolysaccharide（LPS）and to explore it's underlying mechanisms． Methods Cell viability was detected by MTS method; ELISA was used to detect the expression of tumornecrosis factor-α（TNF-α），monocyte chemotactic protein 1（MCP-1），interleukin 1β（IL-1β）and interleukin 6（IL-6）. The changes in protein levels of NF-κB and MAPK signal pathway were detected by Western blot. Results Galangin within 0~20 μmol/L had no effect on cell viability. Concentration of LPS was 500 ng/mL. Compared with the LPS model group，2.5~20 μmol/L of galangin could inhibit the release of TNF-α，MCP-1，IL-1β，IL-6（P＜0.05）. 20 μmol/L of Galangin could inhibit the phosphorylation of IκBα，reduce the occurrence of NF-κB nuclear translocation. However，the protein levels of MAPK signal pathway were unaltered by galangin. Conclusion Galangin has protective effects on THP-1 cells stimulated by LPS. The mechanism of galangin may be related to regulating the NF-κB signal pathway，inhibiting the phosphorylation of IκBα，reducing the occurrence of NF-κB nuclear translocation，thereby inhibiting the release of TNF-α，MCP-1，IL-1β and IL-6.
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Received: 25 December 2015
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Corresponding Authors:
XIONG Xu-ming，E-mail: xiongxuming9@126.com
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