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| Effect of dexamethasone on the expression of aquaporin 1 and 5 in the LPS-induced lung injury in rats |
| CHEN Guo-bing，LIAN Zhu-lan，ZHAN Zhu-qin，WU Jin-zhun，BAI Hai-tao |
| PICU，the First Affiliated Hospital of Xiamen University，Xiamen 361003，China |
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Abstract Objective To investigate the expression changes of Aquaporin（AQP）1，5 in acute lung injury induced by lipopolysaccharide（LPS）and the effect of different doses of dexamethasone on them. Methods Wistar rats were randomly divided into 5 groups: control-group（NC），acute lung injury （ALI）group，3 doses of dexamethasone（DEX）intervention group（small dose 0.5 mg/kg，medium dose 1 mg/kg，large dose 5 mg/kg）. The ALI-group was injected with LPS by tail vein，and the intervention group was given DEX after LPS injection. Rats were sacrificed in 2，6，12 and 24 h，and the samples were collected after the successful modeling. Lung HE-staining morphological changes were observed;lung wet/dry weight ratio（W/D）and lung permeability index were detected; TNF- α，MIP- 1α level were detected by ELISA methods. At the same time，AQP1，AQP5 protein and mRNA content changes were detected through Western blot，immunohistochemistry and real-time PCR. Results Comparedwith control group，the TNF-α，MIP-1α level in ALI group were significantly elevated at 2，6 and 12 h（P＜ 0.01），and gradually down to normal level to 24 h point. Compared with the ALI group ，the inflammatory factors in Dex-intervention group were decreased gradually（P＜0.05）. In ALI-group，alveolar and interstitial edema and inflammatory cell infiltration were observed，12 h was the most obvious but the pathological changes were significantly alleviated in Dex-group. The W/D and lung permeability index were significantly higher in LPS-group than those in the control-group（P＜0.05），and reached the peak in 12 h，while the Dex-intervention group was significantly decreased（P＜0.05），but there was no statistical significance among the three Dex- groups（P＞0.05）. The expression of AQP1 and AQP5 was significantly lower in ALI-group than that in control-group（P＜0.01），and the most obvious decline in 12 h points while the expression of AQPs was significantly increased in Dex-group than that in ALI- group，but there was no statistical significance among the three Dex- groups （P＞0.05）. Conclusion LPS can induce rats acute lung injury. Dexamethasone has a protective effect on lung injury，the mechanism of action may be related to the increase expression of AQP1 and AQP5，while has not dose-effect relationship with dexamethasone.
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Received: 03 June 2015
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Corresponding Authors:
WU Jin-zhun，E-mail: 1923731201@qq.com
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