姜黄素预处理对沙漠干热环境热射病大鼠肺损伤及氧化应激指标变化的影响

doi:10.3969/j.issn.1002-1949.2018.11.016

Chinese Journal of Critical Care Medicine

2018, Vol. 38

Issue (11): 1000-1004 DOI: 10.3969/j.issn.1002-1949.2018.11.016

Effects of curcumin pretreatment on lung injury and oxidative stress index in heatstroke rats under dry heat environment of desert

Li Jia - jia, Ma Na, Tang Jun, Cao Wei, Jiang Jing, Dong Xiang, Shi Wen-hui, Li Jian - ying, Xu Qin, Zhang Dong - hui, Song Lai - yang, Liu Jiang - wei

Key Labortary of the Special Environmental Medicine of Xinjiang, General Hospital of Xinjiang Military Command, Urumqi 830000, China

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Abstract Objective To investigate the changes of the lung tissue damage and oxidative stress in heatstroke rats under dry heat environment of desert by curcumin pretreatment. Methods 50 Sprague-Dawley rats were randomly divided into five groups (n=10): Normal control group (NC group), Dry heat control group (DHC group), Low concentration group (50 mg/kg curcumin pretreatment group), Middle concentration group (100 mg/kg curcumin pretreatment group), High concentration group (200 mg/kg curcumin pretreatment group). The NC and DHC groups were given a gavage of normal saline, while curcumin pretreatment groups were given a gavage of curcumin with the concentration 50 mg/kg, 100 mg/kg, 200 mg/kg, once a day for 7 consecutive days. At 8th day, all except for NC group were transferred to the climate cabin (The Simulated Climate Cabin for Special Environment of Northwest of China) with the conditions of (41±0.5)℃, (10±1)% relative humidity. At the time of 150th minute since the experiment began, the rats were in heat stroke states, and same done to Control group. Lung tissues were harvested and pathological changes were observed by HE staining. Colorimetric determination of MDA, MPO, SOD and CAT levels. Results Lung injury pathological scores were higher in DHC group than in NC group, the lung injury pathological scores gradually decreased with the increased curcumin concentration, and each group had significant different (P<0.01). The content of MDA and MPO in the groups of DHC and curcumin was higher than NC group, and the activity of CAT and SOD decreased. The content of MDA (73.71±1.12, 54.13±1.60, 40.53±1.06) and MPO (5.330.17, 4.30±0.13, 3.75±0.16) in DHC group was significantly increased than those in curcumin groups (P<0.01), and the activity of CAT (2.67±0.04, 2.85±1.00, 3.17±0.09) and SOD (31.834.92, 44.57±5.65, 55.00±5.03) was significantly decreased (P<0.01). Pearson correlation analysis indicated that lung injury pathological scores had positive correlation with MDA and MPO (r=0.945, r=0.962, respectively, P<0.01) and had negative correlation with SOD and CAT (r=-0.903, r=-0.935, respectively, P<0.01). Conclusion Curcumin pretreatment has protective effect on the lung injury in dry heat environment of desert, and the curcumin may exert lung injury protective effects partly through regulating oxidative stress.

Key words： Curcumin Desert Dry heat environment Heat stroke Lung injury Oxidative stress

Corresponding Authors: Liu Jiang - wei, E - mail: ljw273273@163.com

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	Li Jia - jia
	Ma Na
	Tang Jun
	Cao Wei
	Jiang Jing
	Dong Xiang
	Shi Wen-hui
	Li Jian - ying
	Xu Qin
	Zhang Dong - hui
	Song Lai - yang
	Liu Jiang - wei

Cite this article:

Li Jia - jia,Ma Na,Tang Jun, et al. Effects of curcumin pretreatment on lung injury and oxidative stress index in heatstroke rats under dry heat environment of desert[J]. Chinese Journal of Critical Care Medicine, 2018, 38(11): 1000-1004.

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http://www.cnemergency.org.cn/EN/10.3969/j.issn.1002-1949.2018.11.016 OR http://www.cnemergency.org.cn/EN/Y2018/V38/I11/1000

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