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| Composition and variation analysis of the TCR β-chain CDR3 repertoire in the early stage of sepsis using high-throughput sequencing |
| Jiang Yi-chun, Yuan Feng-hua, Zhao Ying, Huang Ze-wei, Liu Zhen-mi, Liu Xiao-jun, Liu Xue-yan |
| Department of ICU, the Second Clinical Medical College (Shenzhen People′s Hospital), Jinan University, Shenzhen 518020, China |
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Abstract Objective To analyze the composition and polymorphism of the T cell receptor（TCR） β-chain complement determining region（CDR3） repertoire in the early stage of sepsis using high-throughput sequencing. Methods 6 cases of sepsis were included in the Intensive Care Unit in Shenzhen People′s Hospital. 9 cases of age-matched volunteers were collected from the Physical Examination Center as control group. The venous blood was collected, the peripheral blood mononuclear cells were isolated and DNA was extracted. The TCR β-chain CDR3 was amplified by multiplex-PCR, and high-throughput sequencing and data analysis of the products were carried out. Results The length of TCR β-chain CDR3 of patients with sepsis and the control group exhibited a Gauss distribution, and there was a significant difference in the expression level of the first 60 specific nucleotide sequences and the specific amino acids between two groups. The expression level of the 59 genes of the TRβV fragment was compared, in which the expression level of TRBV 10-2, TRBV 10-3, TRBV 12-1 and TRBV 5-7 were statistically different (P<0.05). The expression level of 14 genes in TRβJ fragment was analyzed by F test. The expression level of TRBJ 1-1, TRBJ 1-2, TRBJ 2-1 and TRBJ 2-5 had significant differences (P<0.05). Conclusion The polymorphism of TCR β-chain CDR3 in T cells in the early stage of sepsis has been significantly altered, suggesting that it is related to the immune pathogenesis of sepsis, which will provide a new method and means for the pathogenesis, treatment and prognosis of sepsis.
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Corresponding Authors:
Liu Xue-yan, E-mail: liuliu_512@qq.com
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