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| Effect of Apelin-13 on myocardial remodeling in the rabbits with acute myocardial infarction |
| Zhang Bo, Zhang Yan-hong, Chen Li-tao |
| Emergency Internal Medicine, Jilin Provincial People′s Hospital, Changchun 130021, China |
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Abstract Objective To explore the effect of Apelin-13 on myocardial remodeling in the rabbits with acute myocardial infarction (AMI). Methods Rabbit AMI models were established by ligation of open chest coronary artery. They were randomly divided into three groups: sham operation group (n=10), AMI control group (n=10), and Apelin-13 group (n=10). AMI rabbits were given medication after operation, and Apelin-13 was injected by 0.1 mg/（kg·d） through ear vein for 7 consecutive days. AMI control group and sham operation group were injected with the same amount of saline. One or two weeks after operation, 5 mL of vein blood was collected from the ear margin, and 2 weeks later, the animals were sacrificed. Vascular endothelial growth factor (VEGF) in blood was detected, cardiomyocyte apoptosis index (AI) was calculated, heart weight to body weight ratio was obtained, myocardial collagen content was determined and heart samples were fixed for pathological analysis. Results Compared with AMI control group［VEGF after one week (70.59±9.46)ng/L and VEGF after two weeks (59.72±4.17) ng/L, AI (73.97±5.03)%, heart weight and body weight ratio (2.7±0.11)‰, myocardial collagen content (26.85±1.43)mg/g］, VEGF after one week (584.02±5.24)ng/L and VEGF after two weeks (68.65±4.91)ng/L in Apelin-13 group increased significantly(P<0.01), AI decreased significantly ［(65.73±4.42)%, P<0.01］, heart weight and body weight ratio ［(2.60±0.15)‰, P<0.05］, myocardial collagen content decreased significantly ［(24.93±1.37)mg/g, P<0.05］. Special staining showed that in AMI control group, the proliferation of collagen components around blood vessels was obvious. Collagen deposition in Apelin-13 group was significantly reduced than that in AMI control group. Conclusion Apelin-13 promotes neovascularization in AMI rabbits, forms collateral circulation as early as possible, effectively inhibits myocardial apoptosis and improves myocardial remodeling.
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| About author:: Zhang Bo, E-mail:zhb20014@163.com |
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