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| Rat hyperirritable change of HPA axis and pathological observation of cerebral oadema in simulated environment of high altitude and hypoxia |
| LUO Xiao-hong, GUO Wen-jing, NIU Xiao-juan,NIU Ting-xian, FENG Xiao-ming, GUO Xiao-yu |
| Department of Endocrinology，Lanzhou General Hospital，Lanzhou Military Area Command of PLA，Lanzhou 730050, China |
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Abstract Objective To explore the regulation hormone secretion of HPA axis, vascular endothelial growth factor in different hypoxic conditions and the correlation of high altitude cerebral oadema.Methods Ninety-six Wistar rats were randomly divided into four groups, 1500 m (control), 3500 m,5000 m and 6000 m groups; each group was further divided into hypoxia 12 h, 24 h, 48 h subgroups, totally 12 subgroups with 8 rats in each subgroup. The control rats were put in department of animal experiment (Lanzhou, at an altitude of 1500 m); the hypoxia groups were put at different altitudes in hypobaric
chamber. After hypoxia exposure for 12 h, 24 h, 48 h, heart blood was collected and the animals were
then decapitated; brain tissue was collected to determine dry-wet proportion and histopathologic changes were observed with HE staining. The content of CRH, ACTH, GC, VEGF in serum were determined by
ELISA. Real-time PCR was used to detect the expression of HIF-1 and VEGF mRNA. Results Compared with control group, the rats weight decreased and the hypoxia groups showed obvious brain edema; the water content of rat brain tissue increased; the three main indicating hormones of HPA axis (CRH,ACTH, CORT) and VEGF increased with the increasing of altitude; there were statistical significances
(P＜0.05). The expression of HIF-1 mRNA, VEGF mRNA also increased with the increasing of altitude
with statistical significances (P＜0.05). Conclusion With the increasing of altitude, HPA axis stress reaction is abnormally enhanced; increased VEGF level by high altitude is closely related to high altitude cerebral adema. HPA axis may play an important regulating role in the high altitude cerebral oadema invasion and prediction.
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Received: 25 October 2015
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| About author:: LUO Xiao-hong, E－mail: lzfmluo@163.com |
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