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| Detection and clinical significance of serum miR-21 and miR-29a in patients with myocardial ischemia and reperfusion |
| Zhang Jian-xing, Jiang Zhi-zhong |
| Department of Cardiology, the Fifth Affiliated Hospital of Southern Medical University, Guangzhou 510900, China |
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Abstract Objective To investigate the expression and clinical significance of serum miR-21 and miR-29a in patients with myocardial ischemia and reperfusion. Methods We retrospectively analyzed 80 patients with myocardial ischemia-reperfusion in our hospital from May 2016 to December 2017. According to the prognosis of myocardial ischemia reperfusion, patients were divided into prognosis group and poor prognosis group. Twenty healthy volunteers who were examined were included in the control group. The serums of all subjects were collected. The expression levels of miR-21 and miR-29a in the serum were detected by real-time quantitative PCR before operation, 1 day after operation and 5 days after operation. Results The expression levels of miR-21 and miR-29a in serum of patients with myocardial ischemia-reperfusion before and 1 day after operation were significantly higher than those of the control group (both P<0.05), and preoperative miR-21 and miR-29a the expression of 2 groups of myocardial infarction group(miR-21: 7.326±1.694, 6.425±1.344; miR-29a: 7.984±1.264，6.583±2.163)>2 groups of angina pectoris group(miR-21: 3.324±1.695，4.265±0.885; miR-29a: 3.365±1.635, 4.324±1.035), the difference between the groups was statistically significant (P<0.05). The expression of miR-21 and miR-29a was decreased at 1 day after operation, and the expression of miR-21 and miR-29a was increased at 5 days after operation. The difference between the two groups was statistically significant (P<0.05). There was no significant difference in the three time points of the control group (P>0.05). The serum levels of miR-21 and miR-29a in the prognosis group were significantly lower than those in the poor prognosis group (3.493±1.234 vs.6.484±2.135,3.904±1.272 vs.6.954±1.854,P<0.05). Conclusion miR-21 and miR-29a are abnormally expressed in the serum of patients with myocardial ischemia and reperfusion. The combined detection of multiple miRNAs is conducive to the evaluation of the occurrence, diagnosis, treatment and prognosis of myocardial ischemia-reperfusion.
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| About author:: Zhang Jian-xing,E-mail: 993988856@qq.com |
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| ［1］Gao CK, Liu H, Cui CJ, et al. Roles of MicroRNA-195 in cardiomyocyte apoptosis induced by myocardial ischemia-reperfusion injury［J］. J Genet, 2016, 95(1):99-108.
［2］Peralta-Zaragoza O, Deas J, Meneses-Acosta A, et al. Relevance of miR-21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells［J］. BMC Cancer, 2016, 16(1):215.
［3］Garcia R,Nistal JF,Merino D, et al. p-SMAD2/3 and DICER promote pre-miR-21 processing during pressure overload-associated myocardial remodeling［J］. Biochim Biophys Acta, 2015, 1852(7):1520-1530.
［4］Zhao Y, Xu L, Qiao Z, et al. Yi Xin-Shu, a Sheng Mai-San-based traditional Chinese medicine formula,attenuates myocardial ischemia/reperfusion injury by suppressing mitochondrial mediated apoptosis and upregulating liver-X-receptor α［J］. Sci Rep, 2016, 6:23 025.
［5］Gasque Schoof CR, Izzotti A, Jasiulionis MG.The Roles of miR-26, miR-29,and miR-203 in the Silencing of the Epigenetic Machinery during Melanocyte Transformation［J］. Biomed Res Int, 2015, 2015:634 749.
［6］Sandanger, Gao E, Ranheim T, et al. NLRP3 inflammasome activation during myocardial ischemia reperfusion is cardioprotective［J］. Biochem Biophys Res Commun, 2016, 469(4):1012-1020.
［7］Pang JJ, Barton LA, Chen YG, et al. Mitochondrial aldehyde dehydrogenase in myocardial ischemia-reperfusion injury:from bench to bedside［J］. Sheng Li Xue Bao, 2015, 67(6):535-544.
［8］Wang X, Zhong H, Wang L, et al. MiR-29 Induces K562 Cell Apoptosis by Down-Regulating FoxM1［J］. Med Sci Monit, 2015, 21:3115-3120.
［9］Hedegaard ER, Johnsen J, Povlsen JA, et al. Inhibition of KV7 channels protects the rat heart against myocardial ischemia and reperfusion injury［J］. J Pharmacol Exp Ther, 2016, 357(1):94-102.
［10］Wang D, Fan Z, Liu F, et al. Hsa-miR-21 and Hsa-miR-29 in tissue as potential diagnostic and prognostic biomarkers for gastric cancer［J］. Cell Physiol Biochem, 2015, 37(4):1454-1462.
［11］Chen XP, Lu SJ, Huang K, et al. Effects of AngⅡ perfusion on transmural heterogeneous of Cx43 in acute myocardial ischemia reperfusion［J］. Asian Pac J Trop Med, 2016, 9(1):96-99.
［12］Chen S, Hua F, Lu J, et al. Effect of dexmedetomidine on myocardial ischemia-reperfusion injury［J］.Int J Clin Exp Med, 2015, 8(11):21 166-21 172.
［13］Han Q, Zhang HY, Zhong BL, et al. Antiapoptotic effect of recombinant HMGB1 A-box protein via regulation of microRNA-21 in myocardial ischemia-reperfusion injury model in rats［J］. DNA Cell Biol, 2016, 35(4):192-202.
［14］Tong Z, Tang Y, Jiang B, et al. Phosphorylation of nucleolin is indispensable to upregulate miR-21 and inhibit apoptosis in cardiomyocytes［J］. J Cell Physiol, 2018, 12(1):263-267.
［15］Gu H, Liu Z, Li Y, et al. Serum-Derived Extracellular Vesicles Protect Against Acute Myocardial Infarction by Regulating miR-21/PDCD4 Signaling Pathway［J］. Front Physiol, 2018, 9:348.
［16］Wang L, Niu X, Hu J, et al. After Myocardial Ischemia-Reperfusion, miR-29a, and Let7 could affect apoptosis through regulating IGF-1［J］. Biomed Res Int, 2015, 20(15):245 412.
［17］Wu Q, Fang T, Chen M, et al. Endothelial growth medium suppresses apoptosis of mesenchymal stem cells in vitro via decrease of miR-29a［J］. Mol Med Rep, 2017, 16(3):2675-2681. |
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