急性胰腺炎患者外周血α-MSH IL-2 TNF-α及IFN-γ的动态检测及临床意义

doi:10.3969/j.issn.1002-1949.2019.02.009

Chinese Journal of Critical Care Medicine

2019, Vol. 39

Issue (2): 140-144 DOI: 10.3969/j.issn.1002-1949.2019.02.009

Dynamic detection and clinical significance of α-MSH, IL-2, TNF-α and IFN-γ in peripheral blood of patients with acute pancreatitis

Gao Ming, Li He, Ge Wei-wei, Sun Yuan-song, Yin Chun-lin, Wang Hai-ping

Department of Emergency Surgery, Second Affiliated Hospital of Medical University of Anhui, Hefei 230601, China

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Abstract Objective To investigate the dynamic changes of α-melanocyte stimulating hormone (α-MSH), interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in peripheral blood of patients with acute pancreatitis and their clinical significance. Methods We selected 60 cases of acute pancreatitis in emergency surgery of our hospital from January 2016 to January 2018, of which 30 cases of light disease, 30 cases of severe group, and 30 healthy volunteers as control group. All patients were sampled venous blood on the first, third, seventh day after admission, and the levels of α-MSH, IL-2, TNF-α and IFN-γ were measured by ELISA. Results On the first, third, seventh day after admission, the level of serum α-MSH in the light disease group and the severe group decreased gradually, and the level of α-MSH in the severe group had been lower, and the level of α-MSH in the severe group at each time point was significantly lower than that of the light disease group, and the concentration of α-MSH on the first, third, seventh day of the two groups was significantly lower than that in the control group, the difference was statistically significant (P<0.05). On the first, third, seventh day after admission, the serum levels of IL-2 in the light disease group and the severe group increased gradually, and the level of IL-2 in the severe group had been higher, and the level of IL-2 in the severe group was significantly higher than that of the light disease group. At the same time, the concentration of IL-2 in the two groups was significantly higher than that of the control group. The difference was statistically significant (P<0.05). On the 3 and 7 day, the levels of serum TNF-α in the light disease group and the severe group increased gradually, and the level of TNF-α in the severe group had been higher, and the level of TNF-α in the severe group was significantly higher than that of the light disease group. At the same time, the concentration of TNF-α in the two groups was significantly higher than that of the control group. The difference was statistically significant (P<0.05), on the first, third day after admission. The content of serum IFN-γ in the 7 day light group and the severe group gradually decreased, and the IL-γ level in the severe group was lower, and the average IFN-γ level in the severe group was significantly lower than that of the light disease group at all time points, and the concentration of IFN-γ on the first, third, seventh day of the two groups was significantly lower than that of the control group, and the difference was statistically significant (P<0.05). As a longitudinal coordinate, the ROC curve drawn by the 1- specificity as the ordinate shows that the area under the combined curves of α-MSH, IL-2, TNF-α and IFN-γ four are 0.769, 0.697, 0.691, 0.688 and 0.886, respectively. Conclusion The levels of α-MSH, IL-2, TNF-α, and IFN-γ in peripheral blood of patients with acute pancreatitis have different clinical significance in assessing the severity of the patient's condition. The sensitivity, specificity and accuracy of α-MSH in the assessment of the disease are higher than those of the other three indicators. The lower peripheral blood α-MSH content of the patients indicates that the patient is severe in the patient's condition. In addition, the combined detection of the four drugs has better clinical value in assessing the severity of the disease, and is worthy of clinical application.

Key words： Acute pancreatitis (AP) α-melanocyte stimulating hormone (α-MSH) Interleukin-2 (IL-2) Tumor necrosis factor-α (TNF-α) Interferon-γ (IFN-γ)

About author:: Gao Ming, E-mail: E-mail： cs896321@163.com

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	Gao Ming
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	Sun Yuan-song
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	Wang Hai-ping

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Gao Ming,Li He,Ge Wei-wei, et al. Dynamic detection and clinical significance of α-MSH, IL-2, TNF-α and IFN-γ in peripheral blood of patients with acute pancreatitis[J]. Chinese Journal of Critical Care Medicine, 2019, 39(2): 140-144.

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http://www.cnemergency.org.cn/EN/10.3969/j.issn.1002-1949.2019.02.009 OR http://www.cnemergency.org.cn/EN/Y2019/V39/I2/140

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