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| The change and role of T and B lymphocyte subsets in patients with acute pancreatitis |
| Gui Haibo, Du Xiao- gang, Chen Xue- mei |
| Emergency Department, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400042, China |
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Abstract Objective To detect the level of T and B lymphocyte subsets in patients with acute pancreatitis（AP）, and explore the correlation between the level of T and B lymphocyte subsets and the severity of AP. Methods Two hundred cases with acute pancreatitis were enrolled in the test group in the First Affiliated Hospital of Chongqing Medical University from April, 2013 to April, 2015, and 40 healthy volunteers in the control group. The test group was further divided into mild acute pancreatitis group（MAP, 78 cases）, moderately severe acute pancreatitis group（MSAP, 73 cases）and severe acute pancreatitis group（SAP, 49 cases）. T lymphocyte subsets including CD3+, CD4+, CD8+, CD4+ CD25+ reg⁃ ulatory T cell（Treg）and regulatory B cell（Breg）were measured using flow cytometry analysis. IL-10 and IL-4 level were measured using ELISA. Results Compared to the control group, there was a signif⁃ icant decrease in the percentage of CD3+, CD4+ T lymphocyte and Breg in MAP, MSAP and SAP group, especially in MSAP and SAP group（P＜0.05）. Compared to the control group, there was a significant in⁃crease of CD4 +CD25 + Treg in MAP, MSAP and SAP group, especially in SAP group（P＜0.05）. There was a negative correlation between CD3 + , CD4 + and Breg and the severity of acute pancreatitis（P＜ 0.05）, and there was a positive correlation between CD4+ CD25+ Treg and the severity of acute pancreati⁃ tis（P＜0.05）. Conclusion The level of T and B lymphocyte subsets was correlated with the severity of acute pancreatitis.
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Received: 29 December 2015
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Corresponding Authors:
Chen Xue-mei, E-mail:cqmudxg@163.com
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