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| Brain injury after cardiopulmonary resuscitation from cardiac arrest in rats induced by three different methods |
| Jing Ying－xia, Li Xiang－min |
| Department of Emergency, Mawangdui Hospital, People's Hospital of Hunan Province, Changsha 410016, China |
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Abstract Objective To determine the brain injury degree of rats by comparing the expressions of ICAM－1 and VCAM－1 in brain tissue after cardiopulmonary resuscitation from cardiac arrest induced by three different methods. Methods Cardiac arrest animal models of rats were built with the use of three methods including suffocation, chest oppression with 3－kilogram object, and clamping of cardiac plexus. Three groups of rats were respectively sacrificed 3, 6 and 12 hours after resuscitation.The cerebral cortical tissues were harvested to determine ICAM－1 and VCAM－1 expression by immunohistochemical methods. Results Compared with the control group, ICAM－1 and VCAM －1 expressions at the corresponding time points in the experimental groups were significantly increased（P＜0.05）, but the differences in ICAM－1 and VCAM －1 expression among the experimental groups were not significant（P＞0.05）. ICAM －1 and VCAM － 1 contents in brain tissue of rats sacrificed 6h and 12h after resuscitation from cardiac arrest induced by chest oppression with 3－kilogram were higher than in rats sacrificed 3 h after resuscitation（P＜0.05）; for rats resuscitated from cardiac arrest inducedby the clamping of cardiac plexus, ICAM －1 expressions in brain tissue of rats sacrificed 6 h and 12 hafter resuscitation were higher than in rats sacrificed 3 h after resuscitation（P＜0.05）. Conclusion The degree of brain injury caused by three different methods are the basically same. All the three models built by different cardiac arrestinducing methods can be used for the research on brain injury after cardiopul⁃ monary resuscitation.
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Received: 23 August 3016
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Corresponding Authors:
Li Xiang－min, E－mail:lxm8229@126.com
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