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| Protective effects of chicoric acid on LPS-induced H9C2 cells injury and its mechanism |
| Li Yi-ling, Deng Wei, Yin Yan-yan, Yu Shu-chun |
| Department of Anesthesiology, Second Affiliated Hospital of Nanchang University, Nanchang 330006, China |
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Abstract Objective To study the protective effect of chicory acid (CA) on cardiomyocyte injury induced by lipopolysaccharide (LPS) and its mechanism. Methods LPS was used to induce H9C2 injury in rat cardiomyocytes, and the protective effect of different concentrations of CA on cardiomyocytes was evaluated. The cell viability was measured by CCK-8 assay. The release of LDH and ROS in LPS-injured cells was detected by flow cytometry. Western blot was used to detect the expression of SIRT1, NF-κB, IκBα, Caspase-3. Results LPS reduced cell viability, increased ROS and LDH production, increased NF-κB and Caspase-3 expression, and decreased the expression of I-κBα and SIRT1, but all these effects were partially but significantly reversed by CA pretreatment. Conclusion CA can inhibit LPS-induced injury of H9C2 cells through regulating SIRT1/NF-κB signaling pathway.
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Corresponding Authors:
Yu Shu-chun, E-mail: yscdoc@hotmail.com
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