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| The effect and mechanism of exogenous hydrogen sulfide on atherosclerosis in apolipoprotein E-/-mice |
| Yang Qin, Jiang Hong, Wen Yuan, He Long-miao, Zhang Ming-xia, Huang Jun |
| Department of Cardiology, the First Affiliated Hospital, Nanchang University, Nanchang 330006, China |
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Abstract Objective: To investigate the regulation and mechanism of exogenous hydrogen sulfide(H2S) on atherosclerosis in ApoE-/- mice by Toll-like receptor 4(TLR4). Methods: The carotid artery atherosclerotic plaques in ApoE-/- mice model was established; the mice were fed with a high-fat diet and administered GYY4137 for 8 weeks. Lipid and atherosclerotic lesions were measured by oil red O staining. TNF-α, IL-6, IL-1 and IL-10 were detected by ELISA. Protein expression of TLR4, PI3K, Akt, P-Akt were evaluated by Western blot. Results: Compared with the control group, the lipid composition and proinflammatory factors TNF- α, IL-6 and IL-1 increased in the model group, and the inhibitory factor IL-10 decreased, and the expression of TLR4, PI3K and P-Akt protein increased in atherosclerotic plaques (P<0.01). GYY4137 medium and high dose intervention reduced the lipid composition and proinflammatory factors TNF-α, IL-6, IL-1 expression in the model group, increased the inhibitory factor IL-10, decreased the expression of TLR4, PI3K and P-Akt protein (P<0.01). The effect of LY294002 intervention was similar to that of GYY4137. Conclusion: Exogenous H2S can regulate the expression of TLR4 and TNF- α, IL-6, IL-1 and IL-10, and prevent the development of carotid atherosclerotic plaques in ApoE-/- mice, which is related to the PI3K-Akt signaling pathway.
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Received: 01 September 2017
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Corresponding Authors:
Huang Jun, E-mail: junhuang918@163.com
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