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| Protective effects of esmolol on intestinal function in septic rat |
| LI Du-Peng, FANG De-Zhou, WANG Ying-Zhen, ZHANG Bei, ZHEN Ling-Ling, DONG Xiao-Rong, MA Li |
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Abstract Objective To explore the protective effects of short-acting β1 receptor blocker esmolol on intestinal injury in septic rats. Methods Seventy-two male Sprague-Dawley rats were randomly divided into three groups（n＝24 each）, sham operation group（Sham）, sepsis group（CLP）, esmolol group（ES）. Each group was randomly selected six rats for observing their survival time in seven days,and the remaining eighteen rats were randomly divided into three subgroups of 6 h, 12 h and 24 h, with six rats in each subgroup. The Sham group was treated with cecal exploratory surgery, while CLP and ES groups were treated with cecal ligation and puncture（CLP）to establish sepsis model. Catheterization on the left internal jugular vein after the sepsis model was performed. Saline of 1 mL/h was continuously pumped into the Sham and the CLP group by micro pump for 6 h; esmolol dilution of 1 mL/h［15 mg / （kg·
h）］was continuously pumped into the ES group for 6 h. Rats in each subgroup were executed at 6 h, 12 hand 24 h after the operation, respectively. The plasma levels of CA, TNF-α and intestinal tissue DAO, iFABP were measured by ELISA. The pathological changes of intestinal tissues were observed by an optical microscope. Results ①The plasma levels of CA in CLP and ES groups at 6 h after CLP were significantly increased compared with Sham group（P＜0.05）, and the plasma level of CA in ES group increased significantly compared with that in Sham and CLP groups（P＜0.05）. At 12 h after CLP, plasma level of CA in ES group was still significantly increased compared with that in Sham group（P＜0.05）; at 24 h after CLP, plasma level of CA in ES group was increased significantly compared with that in CLP group（P＜0.05）. ②At 6 h,12 h and 24 h after CLP, CLP and ES groups plasma levels of TNF-α was significantly increased compared with Sham group（P＜0.05）, and ES group plasma levels of TNF-α was also significantly lower than the CLP group in each time point（P＜0.05）. ③At 6 h,12 h and 24 h after CLP, CLP and ES groups intestinal tissue levels of DAO and i-FABP were significantly reduced compared with Sham group（P＜0.05）, and ES group intestinal tissue levels of DAO and i-FABP were also significantly higher than the CLP group in each time point（P＜0.05）. ④The pathological damage of Sham group was in the lightest degree, the CLP group in the most serious degree, and the pathological damage of ES group was significantly reduced compared with CLP group and was significantly increased compared with Sham group at all the
time points. ⑤Esmolol is able to prolong the survival time of rats with sepsis and increase the survival time as well. Conclusion Esmolol can promote the secretion of catecholamine, inhibit the release of inflammatory cytokines, protect the intestinal mucosal barrier and alleviate the damage of intestinal tissue.
Besides, it can prolong the survival time of rats with sepsis.
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Received: 14 December 2016
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