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| Effects of PI3 - K / Akt and MAPKs signal pathway in oxidative stress induced HUVECs apoptosis |
| LOU Zhen - kai, LI Xing - guo, ZHANG Fu - hou, DONG Zhong - li, WANG Bing, ZHAO Xue - ling |
| Department of Orthopedics, the First Affiliated Hospital of Kunming Medical University, Kunming 650031,China |
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Abstract Objective To explore the effects of PI3 - K / Akt and MAPKs signal pathway in oxidative stress induced HUVECs apoptosis. Methods Human umbilical vein endothelial cells (HUVECs) are isolated and cultured, then cells are exposed to different concentrations of hydrogen peroxide (H2 O2 ) to establish the oxidative stress injury model. Cells viability is evaluated by MTT assay. The apoptosis and intracellular ROS of HUVECs are detected by TUNEL and Rhodamine 123 staining though flow cytometry. Protein expressions of PI3 - K / Akt and MAPKs signal pathway are tested by Western blot. Results HUVECs ' viability ( OD value) were significantly lower in each H2 O2 stimulation groups compared with control group ( P < 0. 01), and showed no concentration and time correlation ( P > 0. 05 ). 24 hours after 200 μmol / L H2 O2 stimulation, the apoptosis index and intrcellular ROS level of HUVECs were significantly increased compared with control group (P < 0. 05). The protein expressions of p - Akt、 p - c - Jun, p - p38, p - ERK1 / 2 in H2 O2 group were significantly up - regulated compared with control group ( P < 0. 05), and this effects were reversed when pre - incubating cells with antioxidant resveratrol (RES) for 2 h. Conclusion Our data suggest that PI3 - K / Akt and MAPKs signal pathway are possibly involved in oxidative stress induced HUVECs apoptosis.
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Received: 09 November 2015
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Corresponding Authors:
ZHAO Xue - ling, E - mail:zxldoctor@ hotmail. com
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Zhongguancun, 