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| Magnesium isoglycyrrhetate inhibits the over-release of inflammatory mediators in LPS-stimulated macrophages through up-regulating heme oxygenation-1 |
| Zhang Lei, Jiang Lei, Zhang Li-kun, Gong Rui, Pan Shang-ha, Zhao Ming-yan |
| Intensive Care Unit, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China |
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Abstract Objective： To investigate whether magnesium isoglycyrrhetate (MgIG) can inhibit high mobility group box-1 protein (HMGB1) and nitric oxide (NO) release by up-regulating heme oxygenase-1 (HO-1) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Methods： RAW264.7 macrophages stimulated with LPS were used as inflammatory cell models. ①The cells were treated with LPS and different concentrations of MgIG for 24 hours firstly: the control group, LPS group and LPS + MgIG groups (0.01 mg/mL,0.10 mg/mL,0.50 mg/mL and 1.00 mg/mL). Secondly, the cells were randomly divided into the control group, LPS group, LPS + MgIG (1.00 mg /mL) group and LPS + MgIG (1.00 mg/mL) + ZnPPIX (10 μmol/L) group. The level of HMGB1 in the supernatant was measured by ELISA. The concentration of NO in the supernatant was measured by Griess method. The expression of HO-1 and iNOS protein was detected by Western blotting. Results： ①Compared with the control group, LPS significantly increased the levels of HMGB1 and NO in the supernatant (P＜0.05), and increased the expression of HO-1 protein (P＜0.05). Compared with the LPS group, the levels of HMGB1 and NO in the supernatant were both decreased with the increased dose of MgIG (P＜0.05), while the expression of HO-1 was significantly increased with the increased dose of MgIG (P＜0.05).②Compared with Control, LPS also significantly increased the concentration of HMGB1 and NO in supernatant (P＜0.05), and notably increased HO-1 and inducible nitric oxide synthase (iNOS) protein expression (P＜0.05). Compared with the LPS group, the levels of HMGB1 and NO in the supernatant and iNOS protein expression in the cell were all markedly decreased by MgIG 〖JP3〗(1.00 mg/mL) (P＜0.05), while the HO-1 protein expression in the cell was significantly augmented (P＜0.05). However, no differences were detected between the LPS group and LPS + MgIG(1.00 mg/mL)+ZnPPIX(10 μmol/L) (P＞0.05). Conclusion： MgIG inhibits the over-release of HMGB1 and NO and the protein expression of iNOS via up-regulation of HO-1 in LPS-stimulated macrophages.
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Received: 16 May 2017
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Corresponding Authors:
Zhao Ming-yan, E-mail: mingyan1970@163.com
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