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| Protective effects of rhIL-11 for intestinal injury associated with sepsis |
| CHEN Yi-kun，ZHANG Hao，JI Chen-chen，REN Guo-qing. |
| 212001 江苏 镇江，江苏大学附属医院急诊科 |
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Abstract Objective To evaluate the protective effect of recombinant human interleukin - 11（rhIL-11）on
intestinal injury associated with sepsis. Methods Eighty SD rats were divided into rhIL-11
treatment group（30 rats），sepsis group（30 rats），normal group（10 rats）and sham group（10 rats）
randomly. Septic rat model was established by cecal ligation and perforation（CLP）. rhIL-11 treatment group and sham group were given subcutaneous injection of rhIL-11 500 μg/（kg·d）additionally
for 3days. However，rats of sepsis group or normal group were injected with balanced solution
（10 mL/ kg·d）.Samples of intestine were collected at 24 h and 48 h after CLP to perform
histopathological study and molecular analysis. The mRNA expressions of Bax were evaluated by transcription-polymerase chain reaction. The protein expressions of JAK1 in lung were assayed by western
blot. Results ①The injury and the necrosis of small intestinal mucosal were reduced significantly
in treatment group compared with that of sepsis group. ②The expression level of Bax mRNA was lower in treatment group compared with that of sepsis group（P＜0.05）. ③The level of JAK1 protein
expression in the treatment group was observably higher than that of the sepsis，sham，and normal
groups（P＜0.05）. Conclusion rhIL-11 can reduce the intestinal injury associated with sepsis，the
main mechanism maybe rhIL-11 can regulate JAK/STAT signal pathway and reduce the expression of Bax.
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Received: 25 December 2015
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Corresponding Authors:
REN Guo-qing, E-mail: doctorgq@sohu.com
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