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| Effects of dexmedetomidine on multiple organ injury induced by hemorrhagic shock in rats |
| Xia Ming-zhu, Jiang Yuan-xu, Dai Zhong-liang, Li Ya-li |
| Hubei Community Health Service Center, Luohu Hospital Group, Shenzhen 518020, China |
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Abstract Objective To investigate effects of dexmedetomidine on hemorrhagic shock (HS) induced multiple organ injury in rats. Methods Male Sprague-Dawley (SD) rats were randomly divided into three groups (n=8): normal saline control group (NS group); hemorrhage shock group (HS group); HS + dexmedetomidine group (HS +D group). NS group was given saline (5 mL/kg, iv) alone, HS group received hemorrhage and resuscitation; HS +D group was given dexmedetomidine (100 μg/kg, ip) thirty minutes before resuscitation; the arterial catheter was placed in the right carotid artery and then was connected to a pressure transducer for the measurement of mean arterial pressure (MAP). Hemorrhage was performed by withdrawing blood into a heparinized syringe (0.025 mL/g) over 10 minutes to lower MAP to 40 mm Hg to 50 mm Hg, MAP was maintained constantly by further blood drawing or reinfusion as needed for 60 minutes. Then, resuscitation was performed by reinfusing the remaining shed blood supplemented with normal saline (2 times the maximum blood volume drawn) over 10 minutes. The animals were sacrificed at 8 h after resuscitation and arterial blood samples were drawn for the measurement of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), nitric oxide (NO), PaO2, ALT, AST, BUN and Cr. The lung, liver and kidney of all rats were removed for evaluation myeloperxidase (MPO) activity and histological characteristics. Results Compared with NS group, HS resulted in the increase of TNF-α, IL-6, IL-8, NO［(1216.96±97.36) pg/mL, (2686.68±178.65) pg/mL, (998.01±101.42) pg/mL, (546.73±106.22) μmol/L］ in serum, while these parameters ［(428.54±65.48) pg/mL, (1656.31±121.57) pg/mL, (468.19±88.31) pg/mL, (387.34±87.15) μmol/L］in HS +D group was lower than that in HS group (P<0.01). Compared with NS group, HS resulted in the decrease of PaO2 (81±7 mm Hg) and the increase of ALT, AST, BUN, Cr ［(1216.96±97.36) U/L, (2686.68±178.65) U/L, (998.01±101.42)μmol/L, (546.73±106.22) μmol/L］ concentration, while PaO2 ［(95±13) mm Hg］ in HS+D〖JP〗 group was higher than that in HS group and the ALT, AST, BUN, Cr［(126.6±11.3) U/L, (109.4±12.2) U/L, (10.63±4.78) μmol/L, (49.8±6.4) μmol/L］ concentration in HS group were lower than those in NS group (P<0.01). The MPO activity［(5.3±0.6) U/g, (16.7±1.8)U/g, (9.2±1.1) U/g］ of lung, liver, kidney tissue in HS group were higher than those in NS group, while these parameters ［(3.8±0.4) U/g, (12.8±1.2) U/g, (6.8±0.8) U/g］ in HS +D group were lower than those in HS group (P<0.01). Compared with NS group, HS induced marked lung, liver, kidney histological injury, which were less pronounced in HS +D group. Conclusion Dexmedetomidine attenuates lung, liver, kidney injury induced by hemorrhagic shock in rats.
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Corresponding Authors:
Li Ya-li, E-mail: 13613051840@163.com
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