血清miR-29及miR-150对急性肾损伤患者预后的预测价值

doi:10.3969/j.issn.1002-1949.2018.07.004

Chinese Journal of Critical Care Medicine

2018, Vol. 38

Issue (7): 581-585 DOI: 10.3969/j.issn.1002-1949.2018.07.004

Prognostic value of serum miR-29 and miR-150 in patients with acute renal injury

Wen Li-ling, Chen Jia-jia, Liang Hong-li, Fu Ming-chang

Department of Nephrology, the Third People′s Hospital of Hainan Province, Sanya 572000, China

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Abstract Objective To evaluate the prognostic value of serum miR-29 and miR-150 in patients with acute renal injury (AKI). Methods One hundred and twenty-six cases of AKI patients admitted to the Third People′s Hospital of Hainan Province from January 2016 to December 2017, according to the survival of the 28 days were divided into the survival group (82 cases) and death group (44 cases). According to the standard of AKI staging, it was divided into AKI 1 (57 cases), AKI 2 (40 cases) and AKI 3 (29 cases). The serum levels of miR-29 and miR-150 were detected by RT-PCR, and the APACHE Ⅱ score of AKI patients was recorded. The value of miR-29 and miR-150 for evaluating the prognosis of AKI patients was analyzed by the ROC curve. Correlation analysis of serum miR-29, miR-150 level and APACHE Ⅱ score in AKI patients was done with Pearson correlation analysis. Results The expression level of miR-29 (3.14±0.96 vs. 1.93±0.42) and miR-150 (4.26±1.18 vs. 2.05±0.48) in the death group was significantly higher than that in the survival group (t=10.703, 15.106, P<0.001). Serum miR-29 (3.34±1.08 vs. 2.19±0.61 and 1.85±0.34) and miR-150 (3.92±1.15 vs. 2.80±0.91 and 2.55±0.84) expression level in AKI phase 3 were significantly higher than those in AKI phase 2 and AKI phase 1(F=5.124, 7.825, P<0.05). The ROC curve showed that the best cut-off value of serum miR-29 and miR-150 in predicting the prognosis of AKI were 2.74 and 3.83, respectively. The two combination of miR-29 and miR-150［AUC (95%CI): 0.918 (0.852～0.974)］ had better sensitivity and specificity in predicting AKI the prognosis of AKI were 93.6% and 86.5%, respectively. Correlation analysis showed that miR-29 level was positively correlated with miR-150 level (r=0.806, P<0.01), miR-29 and miR-150 levels were positively correlated with APACHE Ⅱ score (r=0.634, r=0.702, P<0.01). Conclusion The expression level of serum miR-29 and miR-150 is related to the severity of patients with AKI, and may be a new target for the treatment of AKI and the prognosis of the patients.

Key words： miR-29 miR-150 Acute renal injury(AKI) Prognostic evaluation

About author:: Wen Li-ling, E-mail:13976825618@163.com

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Cite this article:

Wen Li-ling,Chen Jia-jia,Liang Hong-li, et al. Prognostic value of serum miR-29 and miR-150 in patients with acute renal injury[J]. Chinese Journal of Critical Care Medicine, 2018, 38(7): 581-585.

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http://www.cnemergency.org.cn/EN/10.3969/j.issn.1002-1949.2018.07.004 OR http://www.cnemergency.org.cn/EN/Y2018/V38/I7/581

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