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| Protective effect of minocycline on organs in rats with uncontrolled hemorrhagic shock |
| Zheng Qiang, Xiong Shu-yi, Zheng Cheng-dan， Liu Shi-ping, Wen Tian-ming |
| Department of Emergency, Affiliated Hospital of North Sichuan Medical College, Nanchong 617000, China |
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Abstract Objective To investigate the protective effect of minocycline on organs in rats with uncontrolled hemorrhagic shock and its mechanism.Methods 30 healthy male Sprague Dawley (SD) rats were successfully anesthetized and randomly divided into 3 groups (n=10): sham operation group (blank group), fluid resuscitation group (control group), minocycline group (experimental group). Catheter after the success of the experimental group and the control group was prepared with uncontrolled hemorrhagic shock model. 0～0.5 h blood loss, tail blood loss, mean arterial pressure (MAP), heart rate (HR): 0, 0.5, 1.5, 2.5, 3.5 h, 0.5～1.5 h infusion volume and 24 h mortality were recorded. ELISA method was used to detect 3.5 h and 24 h TNF-α and IL-6. Malondialdehyde (MDA), myeloperoxidase (MPO) level and pathological section in heart, liver and kidney, lung, ileum tissue were detected. To evaluate liver and renal function, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatinine (CREA) were detected by automatic biochemical analyzer. The results were analyzed statistically.Results The survival rate of the experimental group was significantly higher than that of the control group (P<0.05) and 0.5～1.5 h fluid volume of the experimental group were lower than that of the control group (P<0.05). 3.5 h ALT, AST, CREA, TNF-α and IL-6 value of experimental group was significantly lower than that of the control group (P<0.05). ALT, AST, CREA, TNF-α and IL-6 in the experimental group and the control value gradually increased during 3.5～24 h, but the 24 h experimental group was significantly lower than that of the control group (P<0.05). The MAD and MPO values of heart, liver, kidney, lung and ileum in the experimental group were lower than those in the control group (P<0.05). The heart, liver, kidney, lung, ileum tissue pathological slices of control group showed more serious structural damage, blurred, inflammatory cell infiltration than the experimental group, and the liver Suzuki′s score of the experimental group was significantly lower than the control group (P<0.05).Conclusion In uncontrolled hemorrhagic shock rats, minocycline can decrease the inflammatory mediators and cytokines such as IL-6 and TNF-α, inhibition neutrophil production of MAD and MPO, thus inhibiting ischemia-reperfusion on organ tissue and cell injury; regulating inflammatory cell generation function, protection of heart, lung, liver and kidney and the ileum, reduce mortality.
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Corresponding Authors:
Liu Shi-ping, E-mail: liusp456@163.com
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