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| Effect of Jinhong Decoction on platelet aggregation and secretion function in sepsis |
| Ye Miao-qing, Fang Bang-jiang, Song Jing-chun, Zhang Wen, Chen Zhen-yi, Deng Dong |
| Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China |
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Abstract Objective To investigate the effect of Jinhong Decoction on platelet aggregation and secretion in mice with sepsis. Methods C57BL/6 male mice were divided into sham operation group, cecal ligation group and Jinhong Decoction group. Platelet count, platelet aggregation rate, serotonin (5-HT) serum concentration and P-selectin expression rate were detected at 4 h and 72 h after 〖JP2〗operation. Results Four hours after operation, the platelet counts of the cecal ligation group (508.00±98.42)×109/L and Jinhong Decoction group(481.20±38.41)×109/L were significantly lower than those of the sham operation group (784.00±85.97)×10 9/L (P<0.05). Seventy-two hours after operation, the platelet count of Jinhong Decoction group(574.80±78.71)×109/L was significantly higher than that of the cecal ligation group (461.00±64.77)×109/L (P<0.05). Four hours after operation, the platelet aggregation rate in the cecal ligation group was (56.67±0.58)%, which was significantly higher than that in the sham operation group (41.33±1.53)% (P<0.05). The platelet aggregation rate of Jinhong Decoction group (48.00±3.46)% was significantly lower than that of the cecal ligation group (P<0.05). At 72 h postoperatively, the platelet aggregation rate in the cecal ligation group was (12.67±4.04)%, which was significantly lower than that in the sham operation group (43.33±2.89)% (P<0.05). The platelet aggregation rate in Jinhong Decoction group (29.00±5.29)% was significantly higher than that in the cecal ligation group (P<0.05), but still lower than that in the sham operation group (P<0.05). Four hours after operation, the expression rate of P-selectin in the cecal ligation group was (15.53±1.60)%, which was significantly higher than that in the sham operation group (4.01%±1.05)% (P<0.05). The expression rate of P-selectin in Jinhong Decoction group (13.63±1.18)% was significantly lower than that in the cecal ligation group (P<0.05), but still higher than the sham operation group (P<0.05). At 72 h postoperatively, the expression rate of P-selectin in the cecal ligation group was (16.78±1.60)%, which was significantly higher than that in the sham operation group (2.33±0.14)% (P<0.05). The expression rate of P-selectin in Jinhong Decoction group (12.92±2.47)% was significantly lower than the cecal ligation group (P<0.05), but still higher than that in the sham operation group (P<0.05). The 5-HT levels of the cecal ligation group and the Jinhong Decoltion group were both lower than those of the sham operation group at 4 hours and 72 hours after opperation, with statistically significant differences(P<0.05), but there was no statisitically significant difference between the cecal ligation group and the Jinhorg Decoction group(P>0.05). Conclusion Jinhong Decoction can improve the platelet number and aggregation ability in sepsis, and exert anti-inflammatory effect by down-regulating the secretion of P-selectin and α-particle.
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Corresponding Authors:
Fang Bang-jiang, E-mail: fangbji@163.com
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