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| The study on the longer time course of seawater aspiration-induced acute lung injury and stress response |
| Yuan Jia-jia, Zhang Xiao-tong, Bao Yun-ting, Xu Zheng-qing, Shu Yuan-zhao, Wang Zi-wei, Du Bin, Pang Qing-feng |
| Department of Pathophysiology, Medical School of Jiangnan University, Wuxi 214122, China |
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Abstract Objective: To observe the morphological and biochemical index of seawater aspiration-induced acute lung injury from 15 min to 3 d after drowning. Methods: A total of 84 male mice were assigned to 10 groups including normal group(10), non-survival group(22) and near-drowning 15 min(10), 30 min(6),1 h(6), 3 h(6), 6 h(6), 12 h(6), 24 h(6), 72 h(6). Lung wet-to-dry weight ratio, blood gas and biochemical analysis were examined in the normal group, near， drowning 15 min group, and non-survival group. The computed tomography images were obtained using 64 mA 120 kV with a voxel of 1.0 mm. The activity of superoxide dismutase, the content of methane dicarboxylic aldehyde, the content and activity of heme oxygease-1, and the content of aquaporin-1 in the lung tissue were determined. The pulmonary histological changes were observed under light microscope. Results: Compared with normal group, the lung injury in near- drowning 15 min, 30 min, 1 h, 3 h, 6 h groups was more serious, especially in 1 h group. In 1 h group, the level of methane dicarboxylic aldehyde is highest while superoxide dismutase activity was lowest. The expression and enzymatic activity of heme oxygease-1 significantly increased and were sustainable for 3 d. Meanwhile, aquaporin -1 expression increased at peak in 6 h group. Conclusion: The seawater aspiration- induced lung injury can self-repair partly dependent on heme oxygease-1 and aquaporin-1.
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Received: 09 May 2017
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Corresponding Authors:
Pang Qing-feng, E-mail: qfpang@jiangnan.edu.cn
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