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| MnTMPyP protects paraquat-induced lung epithelial-like cell injury through inhibition of oxidative stress and mitochondrial apoptosis pathway |
| Xu Yong-min, Song Chun-qing, Sun Da-zhuang, Wang Rui, Dong Xue-song |
| Department of Emergency, the First Hospital, China Medical University, Shenyang 110001, China |
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Abstract Objective To investigate the protective effect and mechanisms of MnTMPyP (Mn) on paraquat (PQ)- induced lung alveolar epithelial-like cell injury. Methods Lung alveolar epithelial-like cells (A549) were cultured in vitro. Superoxide dismutase mimic Mn was an intervention factor. The experiments were divided into four groups: control group: RPMI 1640 medium was added; Mn group: Mn 10 μmol/L; PQ group: PQ 750 μmol/L; PQ+Mn group: Mn 10 μmol/L was pretreated for 90 min, and then add PQ 750 μmol/L. After each group of cells was treated for 24 h, relevant indicators were detected; the cell survival rate was assessed by MTT assay. Cellular apoptosis, mitochondrial transmembrane potential, ROS were assayed by flow cytometry. The activities of Caspase-3 were assayed by spectrophotometry. Western blotting was used to analyze the expression of proteins, such as Bcl-2, Bax. Results Compared with the control group, there was no statistically significant change in Mn group; in PQ group, the cell viability was decreased, the apoptosis rate was increased, the mitochondrial membrane potential was reduced, the production of ROS was increased, Caspase-3 activity was increased. Western blotting showed that the expression of anti-apoptotic protein Bcl-2 decreased and the expression of pro-apoptotic protein Bax increased in mitochondrial apoptotic pathway; Compared with PQ group, in the Mn+PQ group, the cell viability increased, apoptosis rate decreased, mitochondrial membrane potential increased, production of ROS decreased, Caspase-3 activity decreased. Western blotting showed that the expression of Bcl-2 protein was increased, the expression of Bax protein was decreased. Conclusion MnTMPyP can effectively reduce PQ-induced lung epithelial-like cell injury, and its mechanism is related to antagonism of PQ-induced mitochondrial apoptosis pathway.
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Corresponding Authors:
Dong Xue-song, E-mail:dongxues@163.com
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