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| Protective effect of resveratrol post-treament on myocardial ischemia/reperfusion injury of the rats |
| Li Dan, He Fei, Pang Ke |
| Department of Critical Care Medicine, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China |
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Abstract Objective To observe the protective effects of resveratrol on myocardial ischemia/reperfusion (I/R) injury in SD rats and to explore the potential mechanisms. Methods 80 male SD rats were randomly divided into four groups: sham operation group, I/R group, I/R+ resveratrol group (20 mg/kg), I/R+ resveratrol+LY294002 (5 mg/kg). For establishment of myocardial I/R injury animal models, left anterior descending coronary artery was ligated for 30 min followed by reperfusion for 2 h, and injection of resveratrol (20 mg/kg) or LY294002 (5 mg/kg) before reperfusion 1 min via sublingual vein. TTC staining was for assessing the infarction areas of myocardium. Blood samples and myocardial tissues were collected for detecting the concentration of malondialdehyde (MDA) and superoxide dismutase (SOD); meanwhile, the activity of heme oxygenase-1 (HO-1) was determined; Western blot was used to detect the expression of of PI3K, p-Akt, Nrf2 and HO-1 at protein level in the myocardial tissue. Results Compared to the I/R group, resveratrol post-treatment remarkably decreased the infarction areas of myocardium induced by I/R injury; and significantly reduced the concentration of MDA; but, notably increased the concentration of SOD; meanwhile, resveratrol enhanced the activity of HO-1 (P<0.05); furthermore, the expression of PI3K, p-Akt, Nrf2 and HO-1 at protein levels were all increased after resveratrol post-treatment (P<0.05), which were partly eliminated by PI3K/Akt inhibitor LY294002. Conclusion Resveratrol post-treatment plays protective roles on myocardium induced by I/R injury in SD rats, the mechanisms may be correlated with that resveratrol increased the activity and expression of HO-1 through up-regulating the activity of PI3K/Akt/Nfr2, and then plays the anti- oxidative role of HO-1.
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Corresponding Authors:
He Fei, E-mail: 9881226@qq.com
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| ［1］Cuadrado A, Rojo AI. Heme oxygenase-1 as a therapeutic target in neurodegenerative diseases and brain infections［J］. Curr Pharm Des, 2008, 14(5): 429-442.
［2］Buhrmann C, Popper B, Aggarwal BB, et al. Resveratrol downregualtes inflammatory pathway activated by lymphotoxin α (TNF-α) in articular chondrocytes: comparison with TNF-α［J］. PLoS One, 2017, 12(11): e0 186 993.
［3］RegeSD, Kumar S, Wilson DN et al. Resveratrol protects the brain of obese mice from oxidative damage［J］. Oxid Med Cell Longev, 2013, 2013: 419 092.
［4］Kimura Y, Sumiyoshi M. Resveratrol prevents tumor growth and metastasis by inhibiting lymphangiogenesis and M2 macrophage activation and differentiation in tumor-associated macrophages［J］. Nutr Cancer, 2016, 68(4): 667-678.
［5］Chen ML, Yi L, Zhang Y, et al. Resveratrol attenuates Trimethylamine-N-Oxide (TMAO)-induced atherosclerosis by regulating TMAO synthesis and bile acid metabolism via remodeling of the gut microbiota［J］. MBio, 2016, 7(2): e02210-e02215
［6］Yang L, Zhang Y, Zhu M, et al. Resveratrol attenuates myocardial ischemia/reperfusion injury through up-regulation of vascular endothelia growth factor B［J］. Free Radic Biol Med, 2016, 101: 1-9.
［7］Yang H, Zhang A, Zhang Y, et al. Resveratrol pretreatment protected against cerebral ischemia/reperfusion injury in rats via expansion of T regulatory cells ［J］. J Stroke Cerebrovasc Dis, 2016, 25(8): 1914-1921.
［8］Shimizu K, Miyagi S, Miyazawa K, et al. Resveratrol prevents warm ischemia-reperfusion injury in liver grafts from non-heart-beating donor rats［J］. Transplant Proc, 2016, 48(4): 1221-1225.
［9］Zheng H, Guo H, Hong Y, et al. The effects of age and resveratrol on the hypoxic preconditioning protection against hypoxia-reperfusion injury: studies in rat hearts and human cardiomyocytes［J］. Eur J Cardiothorac Surg, 2015, 48(3): 375-381.
［10］Ren J, Fan C, Chen N, et al. Resveratrol pretreatment attenuates cerebral ischemic injury by upregulating expression of transcription factor Nrf2 and HO-1 in rats［J］. Neurochem Res, 2011, 36(12): 2352-2362.
［11］Cheng XY, Gu XY, Gao Q, et al. Effects of dexmedetomidine postconditioning on myocardial ischemia and the role of the PI3K/Akt-dependent signaling pathway in reperfusion injury［J］. Mol Med Rep, 2016, 14(1): 797-803.
［12］He D, Liu X, Pang Y, et al. Inhibitory effect of resveratrol on ischemia reperfusion-induced cardiocyte apoptosis and its relationship with PI3K-Akt signaling pathway［J］. Zhongguo Zhong Yao Za Zhi, 2012, 37(15): 2323-2326.
［13］Jang HJ, Hong EM, Kim M, et al. Simvastatin induces heme oxygenase-1 via NF-E2-related factor 2 (Nrf2) activation through ERK and PI3K/Akt pathway in colon cancer［J］. Oncotarget, 2016, 7(29): 46 219-46 229. |
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