脓毒症患儿中性粒细胞CD64 CD11b分子表达及临床意义探讨

doi:10.3969/j.issn.1002-1949.2018.10.011

Chinese Journal of Critical Care Medicine

2018, Vol. 38

Issue (10): 885-889 DOI: 10.3969/j.issn.1002-1949.2018.10.011

Expression and clinical significance of CD64 and CD11b in children with sepsis

Lai Qian-kun， Liu Guang-hua, Ye Hong, Wang Shi-biao, Mao Xiao-dan

Department of Pediatrics, Fujian Maternity and Children Health Care Hospital, Fuzhou 350000, China

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Abstract Objective To assess the value of peripheral blood neutrophil CD64 and CD11b for the early diagnosis of child sepsis. Methods 99 cases of research object were divided into three groups, Group 1 with sepsis (n=44), Group 2 with non-sepsis infection (n=33), Group 3 healthy children (n=22). Using flow cytometry to test the median fluorescence intensity (median fluorescence intensity, MFI) of peripheral blood neutrophil CD64 and CD11b and detecting the level of peripheral blood C-reactive protein (CRP). Results The expression of CD64, CD11b, CRP were statistically significant in sepsis group with non-sepsis infection group and healthy children (both P<0.01). Two Logistic regression analysis showed that patients in the highest quartile of CD64 had 4.808-fold risk of sepsis and those in the second quartiles had 3.750 times to have sepsis compared with the lowest one. Patients in the highest quartile of CD11b had 4.600-fold risk of sepsis compared with the lowest one. The diagnostic sensitivity and specificity of sepsis were 91.7%, 72.5%, when the optimal cutoff value of CD64 is 6515MFI using ROC curve, which had higher diagnostic value. The diagnostic sensitivity and specificity of sepsis were 70.8%, 56.9%, when the optimal cutoff value of CD11b is 1667MFI using ROC curve. The diagnostic sensitivity and specificity of sepsis were 97.9%, 84.3%, when combined neutrophil CD64 and CRP. Conclusion The expression of peripheral blood neutrophil CD64 and CD11b increased in children with sepsis. Especially, combination of CD64 and CRP further enhances the sensitivity of the expression and its negative predictive value, which can be used as the early diagnosis of children with sepsis and guide the clinic treatment.

Key words： Sepsis CD64 CD11b Child C-reactive protein

Corresponding Authors: Liu guang-hua, E-mail: liugh1962815@hotmail.com

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	Lai Qian-kun
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	Mao Xiao-dan

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Lai Qian-kun,Liu Guang-hua,Ye Hong, et al. Expression and clinical significance of CD64 and CD11b in children with sepsis[J]. Chinese Journal of Critical Care Medicine, 2018, 38(10): 885-889.

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http://www.cnemergency.org.cn/EN/10.3969/j.issn.1002-1949.2018.10.011 OR http://www.cnemergency.org.cn/EN/Y2018/V38/I10/885

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