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| Blast-induced acute lung injury in rabbits and the protective effect of ulinastatin |
| Li Cheng-en,Hao Jian, Li Shu-wen, Ma Hong-hao, Qian Jun, Li Lu-huan, He Hui- bo, Huang Xin |
| Li Cheng-en,Hao Jian, Li Shu-wen, Ma Hong-hao, Qian Jun, Li Lu-huan, He Hui- bo, Huang Xin |
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Abstract Objective To investigate the changes of NF-κB in the lung tissues of rabbits with blast- induced acute lung injury（ALI）, and study the protective mechanism of ulinastatin（UTI）. Methods The model of ALI in rabbits was induced by blast. All the rabbits were randomly divided into 5 groups: control group（A）, blast- induced ALI group（B）and UTI treatment groups of different doses （C, D, E）. One hour later after blast, the rabbits in group（C, D, E）were intravenously injected with dif⁃ ferent doses of UTI（2.5×104, 5×104, 10×104 U/kg）, while the rabbits in group A and group B were inject⁃ ed with same volume of normal saline. The lung tissues and blood were saved at 24 hours. The NF-κB protein was evaluated by Western blotting, and the NF- κB mRNA was measured by the reverse tran⁃ scription-polymerase chain reaction. The wet to dry weight ratios were determined; TNF- α, IL-6 were determined by ELISA method. The pathological changes of lung tissues were observed under the micro⁃ scope. Results The levels of lung wet to dry weight ratio, NF-κB, TNF-α, IL-6 in group B were sig⁃ nificantly higher than those in group A（P＜0.05）. With the treatment of UTI, the values of wet to dry weight ratio, TNF-α, IL-6, NF-κB protein and mRNA in group（C, D, E）were lower than their mea⁃ sures in group B, and group E was the most significant group（P＜0.05）. Histopathology of lung tissue indicated that pulmonary hemorrhage, edema and inflammation in group（D, E）were better than that in group B. Conclusion UTI efficiently down regulates TNF- α and IL- 6 expression in blast-induced ALI by inhibiting the activation of NF-κB, which may be one of its antiinflammatory actions.
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Received: 29 October 2015
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Corresponding Authors:
Hao Jian, E-mail:jianhao105@163.com
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Zhongguancun, 